Review



pfu dna polymerase  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier
Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    New England Biolabs pfu dna polymerase
    Pfu Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 10198 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pfu+dna+polymerase/DNA+Polymerase+I/pm42127134-377-7-10
    Average 99 stars, based on 10198 article reviews
    pfu dna polymerase - by Bioz Stars, 2026-10
    99/100 stars

    Images

    Related Articles

    Expressing:

    Article Title: Comprehensive kinetic characterization and inhibitor study of Ribonuclease E, a key enzyme of RNA metabolism from Mycobacterium smegmatis.
    Article Snippet: Ribonuclease E (RNase E) in Mycobacterium species is an essential gene that plays a crucial role in RNA metabolism.. This is the first detailed kinetic characterization of recombinant RNase E and its deletion mutants, along with virtual high-throughput ligand screening (vHTS) and in vitro inhibitor studies from any Mycobacterium species.. Recombinant wild-type RNase E and its deletion mutants of Mycobacterium smegmatis (M. smegmatis) were cloned, expressed and purified using Ni+2-NTA affinity chromatography.

    Plasmid Preparation:

    Article Title: Comprehensive kinetic characterization and inhibitor study of Ribonuclease E, a key enzyme of RNA metabolism from Mycobacterium smegmatis.
    Article Snippet: Ribonuclease E (RNase E) in Mycobacterium species is an essential gene that plays a crucial role in RNA metabolism.. This is the first detailed kinetic characterization of recombinant RNase E and its deletion mutants, along with virtual high-throughput ligand screening (vHTS) and in vitro inhibitor studies from any Mycobacterium species.. Recombinant wild-type RNase E and its deletion mutants of Mycobacterium smegmatis (M. smegmatis) were cloned, expressed and purified using Ni+2-NTA affinity chromatography.

    Construct:

    Article Title: Klf10 Regulates the Emergence of Glial Phenotypes During Hypothalamic Development.
    Article Snippet: .. Constructs of Klf10 promoter deletion mutants p916 (−800 to +116 bp), p1511 (−1395 to +116 bp), and p1839 (−1723 to +116 bp) were generated by PCR using a Pfu DNA polymerase (NEB, Ipswich, MA, USA) and specific primers (Table 1). ..

    Article Title: Klf10 Regulates the Emergence of Glial Phenotypes During Hypothalamic Development
    Article Snippet: .. Constructs of Klf10 promoter deletion mutants p916 (−800 to +116 bp), p1511 (−1395 to +116 bp), and p1839 (−1723 to +116 bp) were generated by PCR using a Pfu DNA polymerase (NEB, Ipswich, MA, USA) and specific primers (Table ). ..

    Generated:

    Article Title: Klf10 Regulates the Emergence of Glial Phenotypes During Hypothalamic Development.
    Article Snippet: .. Constructs of Klf10 promoter deletion mutants p916 (−800 to +116 bp), p1511 (−1395 to +116 bp), and p1839 (−1723 to +116 bp) were generated by PCR using a Pfu DNA polymerase (NEB, Ipswich, MA, USA) and specific primers (Table 1). ..

    Article Title: Klf10 Regulates the Emergence of Glial Phenotypes During Hypothalamic Development
    Article Snippet: .. Constructs of Klf10 promoter deletion mutants p916 (−800 to +116 bp), p1511 (−1395 to +116 bp), and p1839 (−1723 to +116 bp) were generated by PCR using a Pfu DNA polymerase (NEB, Ipswich, MA, USA) and specific primers (Table ). ..

    Polymerase Chain Reaction:

    Article Title: Klf10 Regulates the Emergence of Glial Phenotypes During Hypothalamic Development.
    Article Snippet: .. Constructs of Klf10 promoter deletion mutants p916 (−800 to +116 bp), p1511 (−1395 to +116 bp), and p1839 (−1723 to +116 bp) were generated by PCR using a Pfu DNA polymerase (NEB, Ipswich, MA, USA) and specific primers (Table 1). ..

    Article Title: A Specific Sinorhizobium Flagellin Suppresses Legume Nodulation Through Immune Activation
    Article Snippet: .. For the generation of transgenic Arabidopsis lines, the full‐length FLS2 coding sequences from Arabidopsis or soybean were amplified by PCR with Pfu DNA polymerase (M0530S, NEB, Ipswich, USA) using pGWB505‐ AtFLS2 (At5g46330.2), GmFLS2a (Glyma.08g083300.1), and GmFLS2b (Glyma.05 g128200.1) ( FLS2 coding sequences were kindly provided by Dr. Alberto P. Macho) as templates (Wei et al. ). ..

    Article Title: Klf10 Regulates the Emergence of Glial Phenotypes During Hypothalamic Development
    Article Snippet: .. Constructs of Klf10 promoter deletion mutants p916 (−800 to +116 bp), p1511 (−1395 to +116 bp), and p1839 (−1723 to +116 bp) were generated by PCR using a Pfu DNA polymerase (NEB, Ipswich, MA, USA) and specific primers (Table ). ..

    Article Title: Leptospiral dissemination is restrained by liver macrophages through Clec4d-driven capture via C/EBPβ activation.
    Article Snippet: .. Target fragments were amplified by PCR using Pfu DNA polymerase (NEB) with 25 cycles of 95 °C for 15 s, 55 °C for 30 s, and 72 °C for 1 min, followed by gel purification (DNA Gel Extraction Kit, Tiangen). .. Following double digestion with restriction enzymes (37 °C for 1–2 h), fragments were ligated into linearized vectors using T4 DNA Ligase (Fermentas) (16 °C for 0.5–1 h, with a vector:insert molar ratio of approximately 1:3).

    Article Title: A Specific Sinorhizobium Flagellin Suppresses Legume Nodulation Through Immune Activation.
    Article Snippet: .. For the generation of transgenic Arabidopsis lines, the fulllength FLS2 coding sequences from Arabidopsis or soybean were amplified by PCR with Pfu DNA polymerase (M0530S, NEB, Ipswich, USA) using pGWB505- AtFLS2 (At5g46330.2), GmFLS2a (Glyma.08g083300.1), and GmFLS2b (Glyma.05 g128200.1) (FLS2 coding sequences were kindly provided by Dr. Alberto P. Macho) as templates (Wei et al. 2020). ..

    Reverse Transcription:

    Article Title: Isolation of cells in a nanopore sensor array
    Article Snippet: .. Polymerases include, but are not limited to, DNA-dependent DNA polymerases, DNA-dependent RNA polymerases, RNA-dependent DNA polymerases, RNA-dependent RNA polymerases, T7 DNA polymerase, T3 DNA polymerase, T4 DNA polymerase, T7 RNA polymerase, T3 RNA polymerase, SP6 RNA polymerase, DNA polymerase I, Klenow fragment, Thermophilus aquaticus DNA polymerase, Tth DNA polymerase, VentR® DNA polymerase (New England Biolabs), Deep VentR® DNA polymerase (New England Biolabs), Bst DNA Polymerase Large Fragment, Stoeffel Fragment, 90N DNA Polymerase, 90N DNA polymerase, Pfu DNA Polymerase, TfI DNA Polymerase, Tth DNA Polymerase, RepliPHI Phi29 Polymerase, TIi DNA polymerase, eukaryotic DNA polymerase beta, telomerase, TherminatorTM polymerase (New England Biolabs), KOD HiFiTM DNA polymerase (Novagen), KOD1 DNA polymerase, Q-beta replicase, terminal transferase, AMV reverse transcriptase, M-MLV reverse transcriptase, Phi6 reverse transcriptase, HIV-1 reverse transcriptase, novel polymerases discovered by bioprospecting, and polymerases cited in US 2007/0048748, U.S. Pat. ..

    Article Title: Normalization of polymerase activity
    Article Snippet: .. Polymerases include but are not limited to T7 DNA polymerase, T3 DNA polymerase, T4 DNA polymerase, T7 RNA polymerase, T3 RNA polymerase, SP6 RNA polymerase, DNA polymerase 1, Klenow fragment, Thermophilus aquaticus DNA polymerase, Tth DNA polymerase, Vent DNA polymerase (New England Biolabs), Deep Vent DNA polymerase (New England Biolabs), Bst DNA Polymerase Large Fragment, Stoeffel Fragment, 9°N DNA Polymerase, Pfu DNA Polymerase, Tfl DNA Polymerase, RepliPHI Phi29 Polymerase, Tli DNA polymerase, eukaryotic DNA polymerase beta, telomerase, Therminator polymerase (New England Biolabs), KOD HiFi DNA polymerase (Novagen), KOD1 DNA polymerase, Q-beta replicase, terminal transferase, AMV reverse transcriptase, M-MLV reverse transcriptase, Phi6 reverse transcriptase, HIV-1 reverse transcriptase, novel polymerases discovered by bioprospecting, and polymerases cited in US 2007/0048748, U.S. Pat. ..

    Transgenic Assay:

    Article Title: A Specific Sinorhizobium Flagellin Suppresses Legume Nodulation Through Immune Activation
    Article Snippet: .. For the generation of transgenic Arabidopsis lines, the full‐length FLS2 coding sequences from Arabidopsis or soybean were amplified by PCR with Pfu DNA polymerase (M0530S, NEB, Ipswich, USA) using pGWB505‐ AtFLS2 (At5g46330.2), GmFLS2a (Glyma.08g083300.1), and GmFLS2b (Glyma.05 g128200.1) ( FLS2 coding sequences were kindly provided by Dr. Alberto P. Macho) as templates (Wei et al. ). ..

    Article Title: A Specific Sinorhizobium Flagellin Suppresses Legume Nodulation Through Immune Activation.
    Article Snippet: .. For the generation of transgenic Arabidopsis lines, the fulllength FLS2 coding sequences from Arabidopsis or soybean were amplified by PCR with Pfu DNA polymerase (M0530S, NEB, Ipswich, USA) using pGWB505- AtFLS2 (At5g46330.2), GmFLS2a (Glyma.08g083300.1), and GmFLS2b (Glyma.05 g128200.1) (FLS2 coding sequences were kindly provided by Dr. Alberto P. Macho) as templates (Wei et al. 2020). ..

    Amplification:

    Article Title: A Specific Sinorhizobium Flagellin Suppresses Legume Nodulation Through Immune Activation
    Article Snippet: .. For the generation of transgenic Arabidopsis lines, the full‐length FLS2 coding sequences from Arabidopsis or soybean were amplified by PCR with Pfu DNA polymerase (M0530S, NEB, Ipswich, USA) using pGWB505‐ AtFLS2 (At5g46330.2), GmFLS2a (Glyma.08g083300.1), and GmFLS2b (Glyma.05 g128200.1) ( FLS2 coding sequences were kindly provided by Dr. Alberto P. Macho) as templates (Wei et al. ). ..

    Article Title: Leptospiral dissemination is restrained by liver macrophages through Clec4d-driven capture via C/EBPβ activation.
    Article Snippet: .. Target fragments were amplified by PCR using Pfu DNA polymerase (NEB) with 25 cycles of 95 °C for 15 s, 55 °C for 30 s, and 72 °C for 1 min, followed by gel purification (DNA Gel Extraction Kit, Tiangen). .. Following double digestion with restriction enzymes (37 °C for 1–2 h), fragments were ligated into linearized vectors using T4 DNA Ligase (Fermentas) (16 °C for 0.5–1 h, with a vector:insert molar ratio of approximately 1:3).

    Article Title: A Specific Sinorhizobium Flagellin Suppresses Legume Nodulation Through Immune Activation.
    Article Snippet: .. For the generation of transgenic Arabidopsis lines, the fulllength FLS2 coding sequences from Arabidopsis or soybean were amplified by PCR with Pfu DNA polymerase (M0530S, NEB, Ipswich, USA) using pGWB505- AtFLS2 (At5g46330.2), GmFLS2a (Glyma.08g083300.1), and GmFLS2b (Glyma.05 g128200.1) (FLS2 coding sequences were kindly provided by Dr. Alberto P. Macho) as templates (Wei et al. 2020). ..

    Gel Purification:

    Article Title: Leptospiral dissemination is restrained by liver macrophages through Clec4d-driven capture via C/EBPβ activation.
    Article Snippet: .. Target fragments were amplified by PCR using Pfu DNA polymerase (NEB) with 25 cycles of 95 °C for 15 s, 55 °C for 30 s, and 72 °C for 1 min, followed by gel purification (DNA Gel Extraction Kit, Tiangen). .. Following double digestion with restriction enzymes (37 °C for 1–2 h), fragments were ligated into linearized vectors using T4 DNA Ligase (Fermentas) (16 °C for 0.5–1 h, with a vector:insert molar ratio of approximately 1:3).

    Gel Extraction:

    Article Title: Leptospiral dissemination is restrained by liver macrophages through Clec4d-driven capture via C/EBPβ activation.
    Article Snippet: .. Target fragments were amplified by PCR using Pfu DNA polymerase (NEB) with 25 cycles of 95 °C for 15 s, 55 °C for 30 s, and 72 °C for 1 min, followed by gel purification (DNA Gel Extraction Kit, Tiangen). .. Following double digestion with restriction enzymes (37 °C for 1–2 h), fragments were ligated into linearized vectors using T4 DNA Ligase (Fermentas) (16 °C for 0.5–1 h, with a vector:insert molar ratio of approximately 1:3).



    Similar Products

    99
    New England Biolabs pfu dna polymerase
    Pfu Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pfu+dna+polymerase/DNA+Polymerase+I/pm42127134-377-7-10
    Average 99 stars, based on 1 article reviews
    pfu dna polymerase - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    86
    Bioneer Corporation pfu dna polymerase
    Pfu Dna Polymerase, supplied by Bioneer Corporation, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pfu+dna+polymerase/pfu+premix/pm42306519-65-11-15
    Average 86 stars, based on 1 article reviews
    pfu dna polymerase - by Bioz Stars, 2026-10
    86/100 stars
      Buy from Supplier

    99
    New England Biolabs pfu polymerase
    Pfu Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pfu+dna+polymerase/T4+DNA+Ligase/us12606834-885-10-18
    Average 99 stars, based on 1 article reviews
    pfu polymerase - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    86
    Brickell Biotech pfu dna polymerase
    Pfu Dna Polymerase, supplied by Brickell Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pfu+dna+polymerase/dna+polymerase+taq/pmc13168695-224-12-15
    Average 86 stars, based on 1 article reviews
    pfu dna polymerase - by Bioz Stars, 2026-10
    86/100 stars
      Buy from Supplier

    86
    Bioneer Corporation platinum pfu dna polymerase
    Platinum Pfu Dna Polymerase, supplied by Bioneer Corporation, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pfu+dna+polymerase/pfu+premix/pm41599399-148-7-11
    Average 86 stars, based on 1 article reviews
    platinum pfu dna polymerase - by Bioz Stars, 2026-10
    86/100 stars
      Buy from Supplier

    96
    tiangen biotech co pfu polymerase
    Pfu Polymerase, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pfu+dna+polymerase/Pfu+DNA+Polymerase/pmc12895648-74-22-24
    Average 96 stars, based on 1 article reviews
    pfu polymerase - by Bioz Stars, 2026-10
    96/100 stars
      Buy from Supplier

    Image Search Results